Abstract: Objective To investigate the method and mechanism of the venous angiography. Methods Forty SD rats were divided into two groups (A, B), 20 rats for each. Each group then was divided into 4 subgroups (a, b, c, and d). In group A, the rats were perfused with a contrast medium of various concentrations simultaneously through jugular vein and tail vein, whereas the rats in group B were perfused with a contrast medium of various concentrations through the carotid artery. All the rats in both groups underwent radiography simultaneously. The images obtained were processed with Photoshop CS4 to analyze the effects of venography. Skins of 4 New Zealand rabbits underwent radiography after perfusion with the contrast medium to investigate the mechanism of the venous drainage. Results The course and branches of a vein were clearly demonstrated in subgroup Ac, whose venography was achieved with 7% gelatin as the solvent. The accompaniment of the vein and the artery, and their relationship were clearly demonstrated in subgroup Bb, whose systematic arteriography and venography were achieved with 2% gelatin as the solvent. With sufficient pressure and perfusate amount, the main trunks and the communicating branches with a large-diameter in skins of the rabbits were clearly observed. Conclusion Not only the venous perfusion can be performed alone to achieve venography, but also the arterial and venous perfusion be performed at the same time to achieve excellent arteriography and venography simultaneously. To accomplish high-quality venography, the “bypass route”

Key words: Vein, Angiography, Clinical anatomy, Rat, New Zealand rabbit